MARC details
| 000 -LEADER |
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| fixed length control field |
03400nam a22002537a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20190728122349.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
180715b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
MSA |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
616.02 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Abdallah Osama Omar 150681 |
| 245 ## - TITLE STATEMENT |
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| Title |
Molecular characterization of hypervirulent phenotype-associated<br/>traits among K. pneumoniae in Egypt RSPM2.6 // GP // Assoc. Prof. Dr. Mahmoud Tawfick // A.L Bishoy Maher (2018 - 2019) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Giza : |
| Name of publisher, distributor, etc. |
MSA, |
| Date of publication, distribution, etc. |
2019. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
54 p. |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE |
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| Title |
PHARMACY DISTINGUISHED PROJECTS 2019 |
| 500 ## - GENERAL NOTE |
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| General note |
Pharmacy - Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Background and Aim: Klebsiella pneumoniae is a Gram-negative and an enteric opportunistic bacterial<br/>pathogen. It includes classical (cKP) strains which cause hospital-acquired (HA) infections, particularly<br/><br/>among immunocompromised patients, and hypervirulent (hvKP) strains that cause invasive community-<br/>acquired (CA) infections in immunocompetent individuals. The hvKP strains have been attributed to<br/><br/>expression of hypermucoviscous phenotype. Recently, hvKP infections are progressively reported globally<br/>not only for CA infections but also for HA infections with increasing morbidity and mortality rates. This<br/>project aimed to study the prevalence of hypermucoviscous phenotype among clinical isolates of K.<br/>pneumoniae from hospitals in Egypt, and investigate the hypermucoviscosity related factors.<br/>Methods: The isolates were identified phenotypically by various biochemical tests and confirmed<br/>genotypically using a specific primer set. The hypermucoviscosity phenotype was detected by using string<br/>test. Eleven different genes that are potentially associated with the phenotypes of hypermucoviscosity and<br/>hypervirulence were investigated by PCR. The biogram antimicrobial susceptibility was evaluated for the<br/>isolates against thirteen antimicrobial agents using Kirby-Bauer disc diffusion method. The isolates were<br/>tested by ERIC-PCR for fingerprinting. Statistical analysis was conducted using Chi-square.<br/>Results: According to string test, hypermucoviscous isolates were 40% of collected isolates. Antimicrobial<br/><br/>susceptibility study showed that 26% of hypermucoviscous isolates were MDR while 36% of non-<br/>hypermucoviscous isolates were MDR. The PCR assay results for Capsular serotype K2 gene showed that<br/><br/>it is harbored by 68% of hypermucoviscous isolates while 7% only by non-hypermucoviscous isolates.<br/>Statistically there is no significant difference in all tested attributes between the cKP and hvKP isolates<br/>except for K2 gene.<br/>Conclusion: The antimicrobial resistance is not corresponded to the hypermucoviscosity phenotype.<br/>Resistance to carbapenems is a common feature among K. pneumoniae strains in Egypt. K2 gene is highly<br/>related to the hypermucoviscosity phenotype. This study show evidence that the hypermucoviscosity<br/>phenotype is not restricted only to the hypervirulent strains and consequently string test is a poor<br/>identification method for Hypervirulent variant especially in countries with low prevalence like Egypt. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Pneumoniae |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mai Ahmed Hassan EL-Gawish 150139 |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mai Mostafa Mansour Emam 152203 |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yara Mohamed Safwat 150991 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1UVwY4BAOnlAoMmglpjUPzOZVP4QF2xHr/view">https://drive.google.com/file/d/1UVwY4BAOnlAoMmglpjUPzOZVP4QF2xHr/view</a> |
| Public note |
FULL TEXT PRESS HERE |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Distinguished Graduation Projects |
