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The investigation of antiviral mechanisms of chitosan nanoparticles encapsulating curcumin against Hepatitis C Virus genotype – 4a on human hepatoma cell lines اآلليات المضادة للفيروسات من جسيمات ال Chitosan النانوية المغلفة بالكركمين ضد النمط الجيني لفيروس االلتهاب الكبدي سي - 4 أ في خالياالكبدالبشرية \\GP\\Dr. Hossam Taha. ( SPRING 2018 ) Aya Ali Mohamed.

By: Aya Ali Mohamed. 150423.
Contributor(s): Samah Loutfy [External Supervisor].
Material type: TextTextSeries: BIOTECHNOLOGY DISTINGUISHED PROJECTS 2018. Publisher: Giza: MSA University, 2018Description: 130 P. :, 26 CM.Subject(s): -- Medical Biotechnology | antiviral mechanisms | Hepatitis CDDC classification: 615.71 Online resources: FULL TEXT PRESS HERE Summary: Current double and triple treatments with direct acting antivirals (DAAs) were recently reported to cause several side effects and thereby effective, less toxic antiviral agents are still needed. Accordingly, this study aimed at investigating the role of some natural and synthetic antivirals; curcumin, chitosan nanoparticles (CSNPs) and chitosan encapsulating curcumin nanocomposite on HCV-G4 replication in Huh7.5 cells. Viral Replication was quantified in HCV/G4 infected Huh7.5 cells either treated or not with curcumin, chitosan nanoparticles (CSNPs) and chitosan encapsulating curcumin nanocomposite using quantitative real time PCR assay. Nanocomposite (chitosan nanoparticles encapsulating curcumin) was successfully prepared and controlled to the size of 15 nm and charges of 25 mV, characterized by TEM, XRD, FTIR. Curcumin was encapsulated at concentration of 42mg/gm of chitosan nanoparticles. Chitosan nanoparticles at concentration of 100μg/mL showed almost 100% reduction of viral replication whereas the non-toxic dose of curcumin (13 μm) showed 50% reduction in the viral initial titer as detected by real-time PCR assay. Combining both agents in the nanocomposite led to a higher reduction in viral replication. Nanocomposite showed a significant reduction in viral replication when compared to curcumin and chitosan alone which suggests the potential role of nanocomposite as a novel, safe and effective antiviral agent. Keywords: curcumin, chitosan, nanocomposite, HCV – 4.
List(s) this item appears in: Biotechnology D. G. P 2017 / 2018
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GP376BIO2018 (Browse shelf) Available 81846

Current double and triple treatments with direct acting antivirals (DAAs) were
recently reported to cause several side effects and thereby effective, less toxic
antiviral agents are still needed. Accordingly, this study aimed at investigating the
role of some natural and synthetic antivirals; curcumin, chitosan nanoparticles
(CSNPs) and chitosan encapsulating curcumin nanocomposite on HCV-G4
replication in Huh7.5 cells. Viral Replication was quantified in HCV/G4 infected
Huh7.5 cells either treated or not with curcumin, chitosan nanoparticles (CSNPs)
and chitosan encapsulating curcumin nanocomposite using quantitative real time
PCR assay. Nanocomposite (chitosan nanoparticles encapsulating curcumin) was
successfully prepared and controlled to the size of 15 nm and charges of 25 mV,
characterized by TEM, XRD, FTIR. Curcumin was encapsulated at concentration of
42mg/gm of chitosan nanoparticles. Chitosan nanoparticles at concentration of
100μg/mL showed almost 100% reduction of viral replication whereas the non-toxic
dose of curcumin (13 μm) showed 50% reduction in the viral initial titer as detected
by real-time PCR assay. Combining both agents in the nanocomposite led to a higher
reduction in viral replication. Nanocomposite showed a significant reduction in viral
replication when compared to curcumin and chitosan alone which suggests the
potential role of nanocomposite as a novel, safe and effective antiviral agent.
Keywords: curcumin, chitosan, nanocomposite, HCV – 4.

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