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The investigation of antiviral mechanisms of chitosan nanoparticles encapsulating curcumin against Hepatitis C Virus genotype – 4a on human hepatoma cell lines اآلليات المضادة للفيروسات من جسيمات ال Chitosan النانوية المغلفة بالكركمين ضد النمط الجيني لفيروس االلتهاب الكبدي سي - 4 أ في خالياالكبدالبشرية \\GP\\Dr. Hossam Taha. ( SPRING 2018 ) Aya Ali Mohamed.

By: Contributor(s): Material type: TextTextSeries: BIOTECHNOLOGY DISTINGUISHED PROJECTS 2018Publication details: Giza: MSA University, 2018.Description: 130 P. :, 26 CMSubject(s): DDC classification:
  • 615.71 A.A.I 2018
Online resources: Summary: Current double and triple treatments with direct acting antivirals (DAAs) were recently reported to cause several side effects and thereby effective, less toxic antiviral agents are still needed. Accordingly, this study aimed at investigating the role of some natural and synthetic antivirals; curcumin, chitosan nanoparticles (CSNPs) and chitosan encapsulating curcumin nanocomposite on HCV-G4 replication in Huh7.5 cells. Viral Replication was quantified in HCV/G4 infected Huh7.5 cells either treated or not with curcumin, chitosan nanoparticles (CSNPs) and chitosan encapsulating curcumin nanocomposite using quantitative real time PCR assay. Nanocomposite (chitosan nanoparticles encapsulating curcumin) was successfully prepared and controlled to the size of 15 nm and charges of 25 mV, characterized by TEM, XRD, FTIR. Curcumin was encapsulated at concentration of 42mg/gm of chitosan nanoparticles. Chitosan nanoparticles at concentration of 100μg/mL showed almost 100% reduction of viral replication whereas the non-toxic dose of curcumin (13 μm) showed 50% reduction in the viral initial titer as detected by real-time PCR assay. Combining both agents in the nanocomposite led to a higher reduction in viral replication. Nanocomposite showed a significant reduction in viral replication when compared to curcumin and chitosan alone which suggests the potential role of nanocomposite as a novel, safe and effective antiviral agent. Keywords: curcumin, chitosan, nanocomposite, HCV – 4.
List(s) this item appears in: Biotechnology D. G. P 2017 / 2018
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Distinguished Graduation Projects Distinguished Graduation Projects Centeral Library Soft Copy located on library Cataloge GP376BIO2018 (Browse shelf(Opens below)) Available 81846
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GP373BIO2018 Monitoring the anti-cancer potential of gold nanoparticles under the influence of electroporation and ultra-low electromagnetic field (ULEMF) frequencies relative to time on colon cancer cell-lines (HCT-116) مراقبة القدرة المضادة للسرطان للجزئيات النانوية الذهبية تحت تأثير التثقيب الكهربائي والترددات الكهرومغناطيسية المنخفضة )ULEMF )نسبيا للوقت على خاليا سرطان القولون )HCT-116( \\GP\\DR. Riham Mohsen ( SPRING 2018 ) GP374BIO2018 Incidence and risk exposure of nitrate and nitrite of Egyptian fruits and vegetables تأثير و مخاطر التعرض للنترات والنتريت في الفواكه والخضروات المصرية \\GP\\Dr. Osama Saad ( SPRING 2018 ) GP375BIO2018 The Biological activity of Conocarpus erectus extracts and their application as cytotoxic agents\\GP\\Dr. Gehan Safwat ( SPRING 2018 ) GP376BIO2018 The investigation of antiviral mechanisms of chitosan nanoparticles encapsulating curcumin against Hepatitis C Virus genotype – 4a on human hepatoma cell lines اآلليات المضادة للفيروسات من جسيمات ال Chitosan النانوية المغلفة بالكركمين ضد النمط الجيني لفيروس االلتهاب الكبدي سي - 4 أ في خالياالكبدالبشرية \\GP\\Dr. Hossam Taha. ( SPRING 2018 ) GP377BIO2018 Selection, Characterization and Application of Bioemulsifiers Produced by certain Bacteria Isolated from Egyptian Habitats\\GP\\Dr. Ali Diab ( SPRING 2018 ) GP378BIO2018 Incidence of Cytogenetic abnormalities in female infertility\\GP\\ DR. Mona Kamal ( Speing 2018 ) GP379BIO2018 Plasma Renin activity in Egyptian hypertensive patients نشاط بالزما الرنيين المغناطيسى فى مرضى ارتفاع ضغط الدم \\GP\\DR. Mona kamal

Current double and triple treatments with direct acting antivirals (DAAs) were
recently reported to cause several side effects and thereby effective, less toxic
antiviral agents are still needed. Accordingly, this study aimed at investigating the
role of some natural and synthetic antivirals; curcumin, chitosan nanoparticles
(CSNPs) and chitosan encapsulating curcumin nanocomposite on HCV-G4
replication in Huh7.5 cells. Viral Replication was quantified in HCV/G4 infected
Huh7.5 cells either treated or not with curcumin, chitosan nanoparticles (CSNPs)
and chitosan encapsulating curcumin nanocomposite using quantitative real time
PCR assay. Nanocomposite (chitosan nanoparticles encapsulating curcumin) was
successfully prepared and controlled to the size of 15 nm and charges of 25 mV,
characterized by TEM, XRD, FTIR. Curcumin was encapsulated at concentration of
42mg/gm of chitosan nanoparticles. Chitosan nanoparticles at concentration of
100μg/mL showed almost 100% reduction of viral replication whereas the non-toxic
dose of curcumin (13 μm) showed 50% reduction in the viral initial titer as detected
by real-time PCR assay. Combining both agents in the nanocomposite led to a higher
reduction in viral replication. Nanocomposite showed a significant reduction in viral
replication when compared to curcumin and chitosan alone which suggests the
potential role of nanocomposite as a novel, safe and effective antiviral agent.
Keywords: curcumin, chitosan, nanocomposite, HCV – 4.

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