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Cytogenetics study in cases of gender crisis دراسه للعلم الوراثى للخلايا فى ازمات نوع الجنس // GP // DR / Ahmed Aref // Dr. Ola Khalifa // Dr. Osama Zaki( SPRING 2018 ) 152721

By: Emad Adel Bassily ( 152721 ).
Material type: materialTypeLabelBookPublisher: GIZA MSA 2018Description: 89 P 25 CM.Subject(s): -- Cytogenetics -- gender crisis | -- Medical BiotechnologyDDC classification: 668.22 Online resources: FULL TEXT PRESS HERE Summary: Disorder of sex development (DSD) are congenital conditions characterized by atypical development of chromosomal, gonadal, and phenotypic sex, DSD is a rare disease that may affect both male and female with a frequency of 1 in 20,000-25,000 in males, while the female prevalence is 1 in 16,000, as female with disorder in sex development may have uterus but with secondary sexual characters resemble those of males and this is due to the increase levels of the androgen hormone during pregnancy and the karyotyping will be 46 chromosomes that includes 44 autosomal chromosome and XY sex chromosomes instead of XX sex chromosomes of a normal female. This project aims to karyotype three patients one male and two females that was expected to have disorder of sex development, through six steps which starts by set up Harvest that starts by set up step to enable cell growth for 72 hrs. Then followed by harvest of the white blood cells, then Spreading, after that Banding step, Photo, and Karyotyping to detect if there is any gender crisis within this sample or not, for example if there is a female with (XY) instead of (XX) and male with (XX) instead of (XY) in their sex Chromosomes. While the results were positive for the three patients as the two females karyotype was 46, XY and the male karyotype
List(s) this item appears in: BIOTECHNOLOGY Distinguished Graduation Projects - Year 2017/2018
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Distinguished Graduation Projects Distinguished Graduation Projects Soft Copy located on INTERNET GP 382 BIO 2018 (Browse shelf) Available 81848

Disorder of sex development (DSD) are congenital
conditions characterized by atypical development of
chromosomal, gonadal, and phenotypic sex, DSD is a rare disease that may affect
both male and female with a frequency of 1 in 20,000-25,000 in males, while the
female prevalence is 1 in 16,000, as female with disorder in sex development may
have uterus but with secondary sexual characters resemble those of males and this
is due to the increase levels of the androgen hormone during pregnancy and the
karyotyping will be 46 chromosomes that includes 44 autosomal chromosome and
XY sex chromosomes instead of XX sex chromosomes of a normal female. This
project aims to karyotype three patients one male and two females that was
expected to have disorder of sex development, through six steps which starts by
set up Harvest that starts by set up step to enable cell growth for 72 hrs. Then
followed by harvest of the white blood cells, then Spreading, after that Banding
step, Photo, and Karyotyping to detect if there is any gender crisis within this sample
or not, for example if there is a female with (XY) instead of (XX) and male with (XX)
instead of (XY) in their sex Chromosomes. While the results were positive for the
three patients as the two females karyotype was 46, XY and the male karyotype

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