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Antimicrobial Efficacy of Silver Nanoparticles Using Poly (NIPAM) Based Micro-gel Particles as a Drug Delivery System against Enterococcus faecalis (An In-Vitro Study) // MS // Prof. Dr. Manar Yehia Fouda // Dr. Mohamed Yehia Hassan // Dr. Reham Mohsen (2017-2018)

By: Nedda Elsayed.
Material type: materialTypeLabelBookSeries: DENTISTRY DISTINGUISHED PROJECTS 2018. Publisher: Giza MSA 2018Description: 9 P. 30 CM.Subject(s): Dentistry -- Drug Delivery System -- Enterococcus faecalisDDC classification: 616 Online resources: FULL TEXT PRESS HERE Summary: This study aims at comparing the intracanal medication efficacy of poly(NIPAM) when used alone or in combination with silver nanoparticles against Enterococcus faecalis. The secondary aim is to observe the structural integrity of the biofilm on the dentin surface. Methods: The MIC and MBC of p(NIPAM) (X), p(NIPAM) with AgNP (Y), and AgNP (Z) were determined by two-fold serial dilution (0.312 mg/mL - 10 mg/mL). Thirty-nine extracted permanent maxillary teeth were gathered, prepared, and contaminated with E. faecalis. Teeth were split into 4 main groups for exposure; 12 in each X, Y, and Z groups, and 3 teeth as a positive control. Groups X, Y, and Z were split into subgroups based on material exposure time; 1 day and 7 days. Two samples from each subgroup was sectioned longitudinally and prepared for observance under scanning electron microscope. Microbial samples were taken for the rest of the samples. The CFU was counted and compared to the control. The Kruskal-Wallis test compared the study parameters among the groups at 1 day and 7 days (p<0.05). Results: The MBC of the AgNPs was 10 mg/mL. For the teeth, samples showed that the AgNP group had the most significant results after 7 days, with a p-value of 0.03. In the presence of p(NIPAM) constriction of the dentinal tubules was found. Significance: AgNPs have shown to be effective against E. faecalis and further studies is needed on the antibacterial potential of p(NIPAM) on E. faecalis.
List(s) this item appears in: Post-Graduate Projects | Dentistry Distinguished Graduation Projects - Year 2017 / 2018
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Master's degree or PHD Master's degree or PHD Soft Copy located on library Cataloge MS27DENT2018 (Browse shelf) Available 81946-1

MSA Post Graduate Studies

This study aims at comparing the intracanal medication
efficacy of poly(NIPAM) when used alone or in combination
with silver nanoparticles against Enterococcus faecalis. The
secondary aim is to observe the structural integrity of the
biofilm on the dentin surface. Methods: The MIC and MBC of
p(NIPAM) (X), p(NIPAM) with AgNP (Y), and AgNP (Z) were
determined by two-fold serial dilution (0.312 mg/mL - 10
mg/mL). Thirty-nine extracted permanent maxillary teeth were
gathered, prepared, and contaminated with E. faecalis. Teeth
were split into 4 main groups for exposure; 12 in each X, Y,
and Z groups, and 3 teeth as a positive control. Groups X, Y,
and Z were split into subgroups based on material exposure
time; 1 day and 7 days. Two samples from each subgroup was
sectioned longitudinally and prepared for observance under
scanning electron microscope. Microbial samples were taken
for the rest of the samples. The CFU was counted and
compared to the control. The Kruskal-Wallis test compared the
study parameters among the groups at 1 day and 7 days
(p<0.05). Results: The MBC of the AgNPs was 10 mg/mL. For
the teeth, samples showed that the AgNP group had the most
significant results after 7 days, with a p-value of 0.03. In the
presence of p(NIPAM) constriction of the dentinal tubules was
found. Significance: AgNPs have shown to be effective against
E. faecalis and further studies is needed on the antibacterial
potential of p(NIPAM) on E. faecalis.

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