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Investigation of the Anticancer Effect of Azurin Produced from Pseudomonas Aeruginosa دراسة التأثير المضاد للسرطان لمادة Azurin المنتجة من Pseudomonas Aeruginosa \\GP\\DR. Ahmed Nada ( Spring 2018 ) \ Sherry El Fouly.

By: Sherry El Fouly. 154231.
Contributor(s): Hisham Shishtawy [External Supervisor.].
Material type: materialTypeLabelBookPublisher: Giza: MSA, 2018Description: 83 P. :, 26 CM.Subject(s): Pseudomonas AeruginosaOnline resources: FULL TEXT PRESS HERE Summary: Azurin is a low molecular weight protein and member of the Cupredoxin family, it is produced by the bacteria Pseudomonas Aeruginosa, and it is a natural scaffold protein that has antiparasitic, antiviral, and, most notably, anti- cancerous properties. In the course of this study, 5 Pseudomonas Aeruginosa isolates were provided by the University of Mansoura and 4 screening processes took place, including gram stain, gel electrophoresis, SDS- PAGE, and electron microscopy, all in search for the isolate with the optimum azurin producing ability. One isolate was chosen for further testing against the breast cancer cell line MCF-7. Azurin was investigated for its ability to decrease cell viability and increase DNA damage. Azurin was shown to be an anticancer agent which achieves its ability by interacting with multiple targets and interfering in multiple steps in the progression of cancer, such as inhibiting P-Cadherin expression, increasing p53, reducing VEGFR-2 tyrosine kinase activity and interfering in the receptor tyrosine kinase EphB2-mediated signaling process.
List(s) this item appears in: BIOTECHNOLOGY Distinguished Graduation Projects - Year 2017/2018
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Distinguished Graduation Projects Distinguished Graduation Projects Soft Copy located on INTERNET GP412BIO2018 (Browse shelf) Available 81873

Azurin is a low molecular weight protein and member of the
Cupredoxin family, it is produced by the bacteria
Pseudomonas Aeruginosa, and it is a natural scaffold protein

that has antiparasitic, antiviral, and, most notably, anti-
cancerous properties. In the course of this study, 5

Pseudomonas Aeruginosa isolates were provided by the University of Mansoura and

4 screening processes took place, including gram stain, gel electrophoresis, SDS-
PAGE, and electron microscopy, all in search for the isolate with the optimum azurin

producing ability. One isolate was chosen for further testing against the breast
cancer cell line MCF-7. Azurin was investigated for its ability to decrease cell viability
and increase DNA damage. Azurin was shown to be an anticancer agent which
achieves its ability by interacting with multiple targets and interfering in multiple
steps in the progression of cancer, such as inhibiting P-Cadherin expression,
increasing p53, reducing VEGFR-2 tyrosine kinase activity and interfering in the
receptor tyrosine kinase EphB2-mediated signaling process.

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