Pharmacy

Sensitive methods are considered to be essential in the analysis of drug residues in
complex animal tissue matrices. The main hazard of such residues is the development
of antimicrobial resistance that may be transferred to the consumers. Accordingly, the
target of our project is mainly the development of validated, sensitive and simple
chromatographic methods aiming for the determination of trimethoprim (TMP) and
sulfadiazine (SDZ) in their pure forms and in residues of meat tissue. Two methods
were developed and validated, through which a comparative study was performed.
The first method is a RP- liquid chromatographic method while the second one is a
miceller chromatographic method. Validation was performed according to the
International Conference of Harmonization guidelines (ICH).Linearity of the studied
drugs covered the range of 0.5-60 μg/mL for SDZ and 0.5-70 μg/mL for TMP by the
first method and in the range of 0.5-100 μg/mL for both drugs using the second
method. The developed methods showed good recovery, repeatability, and sensitivity.
Moreover, high extraction efficiency was obtained without matrix interference in the
extraction process and in the subsequent chromatographic determination. Statistical
comparison of the obtained results compared with those of a reported method revealed
good agreement and proved that there was no significant difference in the accuracy
and precision between the reported and the two developed methods.