BENEFITS OF LASER USE AS A NEW STANDARD CARE FOR CHRONIC PERIODONTITIS فوائد استخدام الليزر كمعيار جديد فى علاج اللثه // GP // Dr.Faten Sayed Bayoumi // T.A. Arwa Ahmed (2017 - 2018)
- Giza : MSA, 2018.
- 56 p.
- PHARMACY DISTINGUISHED PROJECTS 2018 .
Pharmacy - Microbiology
Periodontal disease is considered one of the main pathologic diseases occurring in humans.it happen with inflammatory reaction producing loss of the teeth because of bone resorption. The aim form this study is Addition of laser light to the practice of periodontology which will give the experienced periodontist a new and efficacious alternative to treat chronic periodontitis. And then we will test antimicrobial activity of a newly extracted oil and compare it's effiency against laser effect. Serial dilution method which is each sample of patient's samples diluted with saline. First 1ml gets from the diseased sample into saline tube and mixes it by pipette. Then take 1 ml from the saline tube into another one and mix it and so on till reach the wanted dilution. Viable cell count method as we use 5 samples for each patient, in sample 1 we implant 2, 3 and 4 dilutions in the blood agar. In sample 2, 3 and 4 we implant the 3and 4 dilutions in the blood agar. In sample 5 we implant the 1 and 2 dilutions in the blood agar. Spread plate method which then we spread 0.3 ml of the diluted sample in the blood agar by the spreader which we sterile it in alcohol. Mentha oil technique which is we use a sterile small glass tube to make a hole to put 4 drops of mentha oil. After that we use metal loops and sterile it in the flam then wait to cool then we remove this blood agar part to put the oil. By using Pasteur pipette we put 4 drops of mentha oil in this hole. LASER treatment with different combinations of power and duration showed statistically significant reduction of gingival bacterial growth compared to baseline level at zero time in 100% of cases (with the exception of LPLT that showed no significant reduction of bacterial growth). LPHT was the most effective one than other categories (LPLT, HPLT and HPHT).The immune status of some patients may have contributed to worsen their gingival inflammation. Two patients with lymphadenopathy and one diabetic patient showed severe inflammation (gingival index = 3).Mentha oil treatment showed reduction of gingival bacterial growth compared to baseline level at zero time in 50% of cases, so that LASER treatment is superior to Mentha oil in reducing bacterial growth. After all these