TY  - BOOK
AU  - Moorhead, Greg
TI  - Protein phosphatase protocols
SN  - 158829711X (alk. paper)
AV  - QP609.P56 P762 2007
U1  - 572.68 22
PY  - 2007///
CY  - Totowa, N.J.
PB  - Humana Press
KW  - Phosphoprotein phosphatases
KW  - Laboratory manuals
KW  - Phosphoprotein Phosphatases
KW  - analysis
KW  - isolation & purification
N1  - Includes bibliographical references and index; Analysis of protein phosphatases / Shirish Shenolikar -- A brief introduction to the protein phosphatase families / Tomas Mustelin -- Small molecule inhibitors of Ser/thr protein phosphatases / Mark Swingle, Li Ni, and Richard E. Honkanen -- Synthesis and use of the protein phosphatase affinity matrices microcystin- and microcystin-biotin-sepharose / Greg Moorhead, Timothy A.J. Haystead, and Carol MacKintosh -- Utilizing protein phosphatase inhibitors to define PP2A as a regulator of ataxia-telangiectasia mutated (ATM) / Aaron A. Goodarzi ... [et al.] -- An automated, fluorescence-based method for continuous assay of PP2A activity / Adam M. Wegner ... [et al.] -- An in vivo assay to quantify stable protein phosphatase 2A (PP2A) heterotrimeric species / Matthew S. Gentry, Richard L. Hallberg, and David C. Pallas -- Mutagenesis and expression of the scaffolding Aa and Ab subunits of PP2A / Ralf Ruediger, Jin Zhou, and Gernot Walter -- Isolation and characterization of PP2A holoenzymes containing FLAG-tagged B subunits / Deanna G. Adams and Brian E. Wadzinski -- Purification of PP2A holoenzymes by sequential immunoprecipitation with anti-peptide antibodies / Gernot Walter, Jin Zhou, and Ralf Ruediger -- Purification of PP2Ac from bovine heart / Hue T. Tran ... [et al.] -- Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions / Laura Trinkle-Mulcahy ... [et al.] -- Yeast two-hybrid screens to identify drosophila PP1-binding proteins / Daimark Bennett and Luke Alphey -- Identification of cellular protein phosphatase-1 regulators / David W. Roadcap, Matthew H. Brush, and Shirish Shenolikar --; Assay for 3-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2 / Masumi Eto and David L. Brautigan -- Phosphorylation of the protein phosphatase type 1 inhibitor protein, CPI-17, by protein kinase C / Michael P. Walsh ... [et al.] -- Purification of smooth muscle myosin phosphatase using a thiophosphorylated myosin light chain-affinity resin / Merideth Borman and Justin MacDonald -- Proteins interacting with saccharomyces cerevisiae type 1 protein phosphatase catalytic subunit identified by single-step affinity purification and mass spectrometry / Edmund P. Walsh ... [et al.] -- Expression of protein histidine phosphatase in Escherichia coli, purification, and determination of enzyme activity / Nicole B©Þumer .. [et al.] -- The use of RNA interference to analyze protein phosphatase function in mammalian cells / Iain Fraser ... [et al.] -- Recognition of a PP2C interaction motif in several plant protein kinases / Niranjan Chakraborty, Ohta Masaru, and Jian-Kang Zhu -- The use of yeast genetic tools to define biological roles of novel protein phosphatases / Joaqu©Ưn Ari©ło ... [et al.] -- Targeting of PP2C in budding yeast / Irene Ota and James Mapes -- Phosphatase targets in TOR signaling / Estela Jacinto -- Functional characterization of the small CTD phosphatases (SCPs) / Michele Yeo and Patrick S. Lin -- Genome-scale discovery and characterization of class-specific protein sequences / David Kerk -- Yeast substrate-trapping system for isolating substrates of protein tyrosine phosphatases / Masahide Fukada and Masaharu Noda; Also available online
UR  - http://ebooks.ohiolink.edu/xtf-ebc/view?docId=tei/sv/9781597452670/9781597452670.xml
ER  -