Pharmacy - Microbiology

Periodontal disease is considered one of the main pathologic diseases
occurring in humans.it happen with inflammatory reaction producing loss
of the teeth because of bone resorption. The aim form this study is Addition
of laser light to the practice of periodontology which will give the
experienced periodontist a new and efficacious alternative to treat chronic
periodontitis. And then we will test antimicrobial activity of a newly
extracted oil and compare it's effiency against laser effect. Serial dilution
method which is each sample of patient's samples diluted with saline. First
1ml gets from the diseased sample into saline tube and mixes it by pipette.
Then take 1 ml from the saline tube into another one and mix it and so on
till reach the wanted dilution. Viable cell count method as we use 5 samples
for each patient, in sample 1 we implant 2, 3 and 4 dilutions in the blood
agar. In sample 2, 3 and 4 we implant the 3and 4 dilutions in the blood
agar. In sample 5 we implant the 1 and 2 dilutions in the blood agar.
Spread plate method which then we spread 0.3 ml of the diluted sample
in the blood agar by the spreader which we sterile it in alcohol. Mentha oil
technique which is we use a sterile small glass tube to make a hole to put
4 drops of mentha oil. After that we use metal loops and sterile it in the
flam then wait to cool then we remove this blood agar part to put the oil.
By using Pasteur pipette we put 4 drops of mentha oil in this hole. LASER
treatment with different combinations of power and duration showed
statistically significant reduction of gingival bacterial growth compared to
baseline level at zero time in 100% of cases (with the exception of LPLT
that showed no significant reduction of bacterial growth). LPHT was the
most effective one than other categories (LPLT, HPLT and HPHT).The
immune status of some patients may have contributed to worsen their
gingival inflammation. Two patients with lymphadenopathy and one
diabetic patient showed severe inflammation (gingival index = 3).Mentha
oil treatment showed reduction of gingival bacterial growth compared to
baseline level at zero time in 50% of cases, so that LASER treatment is
superior to Mentha oil in reducing bacterial growth. After all these