000			01920nam a22002057a 4500
999			_c13994 _d13994
003			OSt
005			20190307091036.0
008			180807b xxu||||| |||| 00| 0 eng d
040			_cMSA.
100			_aSherry El Fouly. 154231
245			_aInvestigation of the Anticancer Effect of Azurin Produced from Pseudomonas Aeruginosa دراسة التأثير المضاد للسرطان لمادة Azurin المنتجة من Pseudomonas Aeruginosa \\GP\\DR. Ahmed Nada ( Spring 2018 ) \ _cSherry El Fouly.
260			_aGiza: _bMSA, _c2018.
300			_a83 P. :, _b26 CM.
440			_aBIOTECHNOLOGY DISTINGUISHED PROJECTS 2018
520			_aAzurin is a low molecular weight protein and member of the Cupredoxin family, it is produced by the bacteria Pseudomonas Aeruginosa, and it is a natural scaffold protein that has antiparasitic, antiviral, and, most notably, anti- cancerous properties. In the course of this study, 5 Pseudomonas Aeruginosa isolates were provided by the University of Mansoura and 4 screening processes took place, including gram stain, gel electrophoresis, SDS- PAGE, and electron microscopy, all in search for the isolate with the optimum azurin producing ability. One isolate was chosen for further testing against the breast cancer cell line MCF-7. Azurin was investigated for its ability to decrease cell viability and increase DNA damage. Azurin was shown to be an anticancer agent which achieves its ability by interacting with multiple targets and interfering in multiple steps in the progression of cancer, such as inhibiting P-Cadherin expression, increasing p53, reducing VEGFR-2 tyrosine kinase activity and interfering in the receptor tyrosine kinase EphB2-mediated signaling process.
650			_aPseudomonas Aeruginosa.
700			_aHisham Shishtawy. _eExternal Supervisor.
856			_uhttps://goo.gl/uFu8tU _zFULL TEXT PRESS HERE
942			_2ddc _cGP