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008 130823s2013 nyua b 000 0 eng c
010 _a 2012277650
020 _a9780791860090
020 _a0791860094
020 _a1606504274
020 _a9781606504277
035 _a(OCoLC)ocn828888913
040 _aBTCTA
_beng
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050 0 0 _aTP248.27.M53
_bG46 2013
082 _a660.62
100 _aDelvigne, Frank.
245 0 0 _aGFP whole cell microbial biosensors :
_bscale-up and scale-down effects on biopharmaceutical processes /
_cFrank Delvigne ... [et al.].
246 3 _aGreen fluorescent protein whole cell microbial biosensors :
_bscale-up and scale-down effects on biopharmaceutical processes
250 _a1st ed.
260 _aNew York, N.Y. :
_bASME Press :
_bMomentum Press,
_c2013.
264 _c2013
300 _a43 p. :
_bill. (some col.) ;
_c24 cm.
490 1 _aBiomedical & nanomedical technologies - concise monograph series
504 _aIncludes bibliographical references (p. [36]-43).
505 0 _aInteraction between fluid flow and microbial cells : importance of the operating scale -- Stochastic simulation of the displacement of microbial cells along concentration field -- Experimental results gained from the physiological response of GFP biosensors in scale-down conditions -- Another source of information : protein leakage and the study of the secretome.
520 3 _aTwo strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical engineering improvement of the bioreactors and scaling-up rules. This work is more particularly dedicated to this second class of parameters. Recent developments in bioreactor technologies follow the scaling-out principle, i.e. carrying out several cultures in parallel with controlled conditions for screening purposes. Several mini-bioreactor concepts, i.e. bioreactor with working volume of 1 to 100 mL with controlling devices, have been developed following this principle. In general, chemical engineering similarities between conventional stirred bioreactors and their miniature equivalent are well characterized. However, the actual scaling-up rules are not able to cope with the complexity of the microbial stress response. Indeed, microbial stress response still remains not completely understood considering the process perturbations and the environmental fluctuations accompanying the scaling-up to industrial bioreactors. At this time, this kind of response can only be experimentally predicted by using scale-down bioreactors, i.e. lab-scale bioreactors designed in order to reproduce mixing imperfections that have to be expected at large-scale. However, the use of such an approach is time consuming and requires an experimented staff to elaborate the scaling-down protocols. Indeed, bioprocess development involves several steps which cannot be necessarily linked with each other considering the different cultivation equipment used--
650 0 _aMicrobial biotechnology.
650 0 _aGreen fluorescent protein.
650 0 _aBiosensors.
700 1 _aDelvigne, Frank.
830 0 _aBiomedical & nanomedical technologies.
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