Azurin is a low molecular weight protein and member of the
Cupredoxin family, it is produced by the bacteria
Pseudomonas Aeruginosa, and it is a natural scaffold protein

that has antiparasitic, antiviral, and, most notably, anti-
cancerous properties. In the course of this study, 5

Pseudomonas Aeruginosa isolates were provided by the University of Mansoura and

4 screening processes took place, including gram stain, gel electrophoresis, SDS-
PAGE, and electron microscopy, all in search for the isolate with the optimum azurin

producing ability. One isolate was chosen for further testing against the breast
cancer cell line MCF-7. Azurin was investigated for its ability to decrease cell viability
and increase DNA damage. Azurin was shown to be an anticancer agent which
achieves its ability by interacting with multiple targets and interfering in multiple
steps in the progression of cancer, such as inhibiting P-Cadherin expression,
increasing p53, reducing VEGFR-2 tyrosine kinase activity and interfering in the
receptor tyrosine kinase EphB2-mediated signaling process.